UTR Library Designer Results

Template 5'-UTR Sequence (up to 25NT) : NNNNNNNNNNAAAGGAGCATCNNNN,     Additional Constraints for designing 5'-UTR sequence (25NT) : NNNNNNNNNNNNNNNNNNNNNNNNN

Final Constraints for designing 5'-UTR sequence (25NT) : TTTCTTCGCCAAAGSAGSWWCGCGC

Protein Coding Sequence (at least N-term 35NT) : ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT

Number of Trials : 142

16s rRNA Sequence : ACCUCCUUA

tAI (N-term 35NT in Coding Sequence) : 0.25

Desired Expression Range : 200000.00 ~ 40.00

Resolution : 16

Library Sequence : TTTCTTCGCCAAAGSAGSWWCGCGCATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT

Number of Generated Sequences : 16

Start Spacinge 5'-UTR Sequence Protein Coding Sequence tAI dGUTR Expression Level
25 6 TTTCTTCGCCAAAGGAGGTACGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 -6.89 375006.67
 
25 6 TTTCTTCGCCAAAGGAGGTTCGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 -5.89 214357.21
 
25 6 TTTCTTCGCCAAAGGAGGATCGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 -5.09 137030.54
 
25 6 TTTCTTCGCCAAAGGAGGAACGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 -5.09 137030.54
 
25 6 TTTCTTCGCCAAAGGAGCAACGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 -1.79 21639.36
 
25 6 TTTCTTCGCCAAAGGAGCATCGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 -1.39 17301.51
 
25 6 TTTCTTCGCCAAAGGAGCTACGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 0.41 6322.12
 
25 6 TTTCTTCGCCAAAGCAGGTACGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 1.41 3613.78
 
25 6 TTTCTTCGCCAAAGCAGGAACGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 1.61 3231.33
 
25 6 TTTCTTCGCCAAAGCAGGTTCGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 1.66 3142.22
 
25 6 TTTCTTCGCCAAAGGAGCTTCGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 1.71 3055.56
 
25 6 TTTCTTCGCCAAAGCAGGATCGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 1.91 2732.19
 
25 6 TTTCTTCGCCAAAGCAGCAACGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 6.21 246.63
 
25 6 TTTCTTCGCCAAAGCAGCATCGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 6.61 197.19
 
25 6 TTTCTTCGCCAAAGCAGCTACGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 8.41 72.05
 
25 6 TTTCTTCGCCAAAGCAGCTTCGCGC ATGGCTAGCAAGGGCGAGGAGCTGTTCACCGGGGT 0.25 9.71 34.82