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UTR Library Designer Error Template 5'-UTR Sequence (25NT) : NNNNNNNNNNAAAGGAGCATCNNNN , Additional Constraints for designing 5'-UTR sequence (25NT) : NNNNNNNNNNNNNNNNNNNNNNNNN Protein Coding Sequence (at least N-term 35NT) : TAC AGA CTA GTA TCT CCT TAT TAA AGT TAA ACA AAA TTA TTT CTA CAG GGG AAT TGT TAT CCG CTC ACA ATT CCA ( CDS should start with ATG or GTG ) 16s rRNA Sequence : ACCUCCUUA Desired Expression Range : 1002.55 ~ 102672.55 Number of expression-level intermediates : 16 |