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UTR Library Designer Error Template 5'-UTR Sequence (25NT) : NNNNNNNNNNAAAGGAGCATCNNNN , Additional Constraints for designing 5'-UTR sequence (25NT) : NNNNNNNNNNNNNNNNNNNNNNNNN Protein Coding Sequence (at least N-term 35NT) : ATGAAAAACA TCAATCCAAC GCAGACCGCT GCCTGGCAGG CACTACAGAA ACACTTCGAT 61 GAAATGAAAG ACGTTACGAT CGCCGATCTT TTTGCTAAAG ACGGCGATCG TTTTTCTAAG 121 TTCTCCGCAA CCTTCGACGA TCAGATGCTGGTGGATTACT CCAAAAACCG CATCACTGAA 181 GAGACGCTGG CGAAATTACA GGATCTGGCG AAAGAGTGCG ATCTGGCGGG CGCGATTAAG 241 TCGATGTTCT CTGGCGAGAA GATCAACCGC ACTGAAAACC GCGCCGTGCT GCACGTAGCG 301 CTGCGTAACC GTAGCAATAC CCCGATTTTG GTTGATGGCA AAGACGTAAT GCCGGAAGTC 361 AACGCGGTGC TGGAGAAGAT GAAAACCTTC TCAGAAGCGA TTATTTCCGG TGAGTGGAAA 421 GGTTATACCG GCAAAGCAAT CACTGACGTA GTGAACATCG GGATCGGCGG TTCTGACCTC 481 GGCCCATACA TGGTGACCGA AGCTCTGCGT CCGTACAAAA ACCACCTGAA CATGCACTTT 541 GTTTCTAACG TCGATGGGAC TCACATCGCG GAAGTGCTGA AAAAAGTAAA CCCGGAAACC 601 ACGCTGTTCT TGGTAGCATC TAAAACCTTC ACCACTCAGG AAACTATGAC CAACGCCCAT 661 AGCGCGCGTG ACTGGTTCCT GAAAGCGGCA GGTGATGAAA AACACGTTGC AAAACACTTT 721 GCGGCGCTTT CCACCAATGC CAAAGCCGTT GGCGAGTTTG GTATTGATAC TGCCAACATG 781 TTCGAGTTCT GGGACTGGGT TGGCGGCCGT TACTCTTTGT GGTCAGCGAT TGGCCTGTCG 841 ATTGTTCTCT CCATCGGCTT TGATAACTTC GTTGAACTGC TTTCCGGCGC ACACGCGATG 901 GACAAGCATT TCTCCACCAC GCCTGCCGAG AAAAACCTGC CTGTACTGCT GGCGCTGATT 961 GGCATCTGGT ACAACAATTT CTTTGGTGCG GAAACTGAAG CGATTCTGCC GTATGACCAG 1021 TATATGCACC GTTTCGCGGC GTACTTCCAG CAGGGCAATA TGGAGTCCAA CGGTAAGTAT 1081 GTTGACCGTA ACGGTAACGT TGTGGATTAC CAGACTGGCC CGATTATCTG GGGTGAACCA 1141 GGCACTAACG GTCAGCACGC GTTCTACCAG CTGATCCACC AGGGAACCAA AATGGTACCG 1201 TGCGATTTCA TCGCTCCGGC TATCACCCAT AACCCGCTCT CTGATCATCA CCAGAAACTG 1261 CTGTCTAACT TCTTCGCCCA GACCGAAGCG CTGGCGTTTG GTAAATCCCG CGAAGTGGTT 1321 GAGCAGGAAT ATCGTGATCA GGGTAAAGAT CCGGCAACGC TTGACTACGT GGTGCCGTTC 1381 AAAGTATTCG AAGGTAACCG CCCGACCAAC TCCATCCTGC TGCGTGAAAT CACTCCGTTC 1441 AGCCTGGGTG CGTTGATTGC GCTGTATGAG CACAAAATCT TTACTCAGGG CGTGATCCTG 1501 AACATCTTCA CCTTCGACCA GTGGGGCGTG GAACTGGGTA AACAGCTGGC GAACCGTATT 1561 CTGCCAGAGC TGAAAGATGA TAAAGAAATC AGCAGCCACG ATAGCTCGAC CAATGGTCTG 1621 ATTAACCGCT ATAAAGCGTG GCGCGGTTAA 16s rRNA Sequence : ACCUCCUUA Desired Expression Range : 1002.55 ~ 1000000.55 ( Desired max expression should be smaller than 1000000 ) Number of expression-level intermediates : 32 |