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UTR Library Designer Error Template 5'-UTR Sequence (25NT) : NNNNNNNNNNAAAGGAGCATCNNNN , Additional Constraints for designing 5'-UTR sequence (25NT) : YYYYYYYYYYAAAGGAGCATCYYYY Protein Coding Sequence (at least N-term 35NT) : MTEDNIAPITSVKVVTDKCTYKDNELLTKYSYENAVVTKTASGRFDVTPTVQDYVFKLDLKKPEKLGIMLIGLGGNNGSTLVASVLANKHNVEFQTKEGVKQPNYFGSMTQCSTLKLGIDAEGNDVYAPFNSLLPMVSPNDFVVSGWDINNADLYEAMQRSQVLEYDLQQRLKAKMSLVKPLPSIYYPDFIAANQDERANNCINLDEKGNVTTRGKWTHLQRIRRDIQNFKEENALDKVIVLWTANTERYVEVSPGVNDTMENLLQSIKNDHEEIAPSTIFAAASILEGVPYINGSPQNTFVPGLVQLAEHEGTFIAGDDLKSGQTKLKSVLAQFLVDAGIKPVSIASYNHLGNNDGYNLSAPKQFRSKEISKSSVIDDIIASNDILYNDKLGKKVDHCIVIKYMKPVGDSKVAMDEYYSELMLGGHNRISIHNVCEDSLLATPLIIDLLVMTEFCTRVSYKKVDPVKEDAGKFENFYPVLTFLSYWLKAPLTRPGFHPVNGLNKQRTALENFLRLLIGLPSQNELRFEERLL ( CDS should start with ATG or GTG ) 16s rRNA Sequence : ACCUCCUUA Desired Expression Range : 999999.00 ~ 999999.00 ( Desired max expression should be larger than that of min ) Number of expression-level intermediates : 16 |