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UTR Library Designer Error Template 5'-UTR Sequence (25NT) : AAACCAGTACCTTCGGGTAAGAGAT , Additional Constraints for designing 5'-UTR sequence (25NT) : NNNNNNNNNNNNNNNNNNNNNNNNN Protein Coding Sequence (at least N-term 35NT) : ATGACAATTGGGATTGATAAAATTAGTTTTTTTGTGCCCCCTTATTATATTGATATGACGGCACTGGCTGAAGCCAGAAATGTAGACCCTGGAAAATTTCATATTGGTATTGGGCAAGACCAAATGGCGGTGAACCCAATCAGCCAAGATATTGTGACATTTGCAGCCAATGCCGCAGAAGCGATCTTGACCAAAGAAGATAAAGAGGCCATTGATATGGTGATTGTCGGGACTGAGTCCAGTATCGATGAGTCAAAAGCGGCCGCAGTTGTCTTACATCGTTTAATGGGGATTCAACCTTTCGCTCGCTCTTTCGAAATCAAGGAAGCTTGTTACGGAGCAACAGCAGGCTTACAGTTAGCTAAGAATCACGTAGCCTTACATCCAGATAAAAAAGTCTTGGTTGTAGCAGCAGATATTGCAAAATATGGATTAAATTCTGGCGGTGAGCCTACACAAGGAGCTGGGGCGGTTGCAATGTTAGTTGCTAGTGAACCGCGCATCTTGGCTTTAAAAGAGGATAATGTGATGCTGACGCAAGATATCTATGACTTTTGGCGTCCAACAGGCCATCCGTATCCTATGGTCGATGGTCCTTTGTCAAACGAAACCTACATCCAATCTTTTGCCCAAGTCTGGGATGAACATAAAAAAAGAACCGGTCTTGATTTTGCAGATTATGATGCTTTAGCGTTCCATATTCCTTACACAAAAATGGGCAAAAAAGCCTTATTAGCAAAAATCTCCGACCAAACTGAAGCAGAACAGGAACGAATTTTAGCCCGTTATGAAGAAAGCATCATCTATAGTCGTCGCGTAGGAAACTTGTATACGGGTTCACTTTATCTGGGACTCATTTCCCTTTTAGAAAATGCAACGACTTTAACCGCAGGCAATCAAATTGGGTTATTCAGTTATGGTTCTGGTGCTGTCGCTGAATTTTTCACTGGTGAATTAGTAGCTGGTTATCAAAATCATTTACAAAAAGAAACTCATTTAGCACTGCTAGATAATCGGACAGAACTTTCTATCGCTGAATATGAAGCCATGTTTGCAGAAACTTTAGACACAGATATTGATCAAACGTTAGAAGATGAATTAAAATATAGTATTTCTGCTATTAATAATACCGTTCGCTCTTATCGAAACTAA 16s rRNA Sequence : ACCUCCUUA Desired Expression Range : 3000.55 ~ 1000000.59 ( Desired max expression should be smaller than 1000000 ) Number of expression-level intermediates : 8 |