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UTR Library Designer Error Template 5'-UTR Sequence (25NT) : TTATTTACTGCTTTTCTTTTTCACAACCGG ( Length should be 25NT ) , Additional Constraints for designing 5'-UTR sequence (25NT) : YYYYYYYYYYAAAGGAGCATCYYYY Protein Coding Sequence (at least N-term 35NT) : ATGAAAAAACAAAACGACATTCCACAGCCAATCAGAGGAGATAAGGGAGCGACAGTGAAA ATCCCGCGTAATATTGAAAGGGACCGGCAGAATCCCGATATGCTCGTACCGCCGGAAACC GATCATGGCACCGTTGAAAATATGAAGTTTTCTTTCTCAGATACTCATAACCGTTTGGAA AAAGGCGGGTACGCCCGTGAAGTTACCGTACGTGAGCTGCCGATTTCAGAAAACCTGGCT TCTGTTAATATGCGGCTGAAGCCAGGCGCCATTCGAGAGCTTCATTGGCATAAGGAAGCA GAGTGGGCTTATATGATCTACGGGAAAGCACGCGTCACGATTGTGGATGAAAAGGGCCGC AGCTTTATTGATGATGTAGGCGAAGGAGATTTATGGTACTTCCCGTCAGGCCTGCCGCAC TCGATTCAAGCGCTTGAAGAAGGGGCAGAGTTCCTGCTCGTGTTTGACGACGGGTCATTC TCTGAAAACAGCACGTTCCAGCTGACAGACTGGCTTGCCCACACCCCTAAAGAAGTGATT GCCGCTAATTTTGGCGTAACAGAAGAAGAAATTGCCAATTTGCCAGGCAAAGAAAAATAT ATATTTGAAAAACCTGTGCCCGGCTGTTTAAAAGATGACATTGTGGAAGGGCCGTACGGC GAAGTGCCTTATCCGTTTACGTACCGCCTTCTTGAACAGGAGCCGATTGCATCAGAAGGG GGAAAGGTGTACATTGCGGATTCGACAAACTTTACAGTGTCTAAAACCATTGCATCTGCG CTTGTAACCGTGGAACCAGGCGCCATGAGAGAGCTTCACTGGCATCCGAATACGCATGAA TGGCAATATTACATCTCCGGTAAAGCGAGAATGACTGTTTTTGCTGCTGACGGCCATGCG AGAACCTTTAACTATCAAGCCGGTGATGTCGGTTATGTTCCATTTGCGATGGGCCACTAC GTAGAAAACCTCGGCGATGAACCGCTCGTCTTTTTAGAAATCTTTAAAGATGACCATTAT GCCGATGTGTCTTTAAACCAATGGCTCGCAATGCTTCCTGAAACCTTCGTCCAAGAACAC CTTGACTTAGGCAAAGAATTTACGGATGTCCTTTCAAAAGAAAAGCATCCGGTTGTGAAA AAGAAAAGCAGTAAATAA 16s rRNA Sequence : TTATCGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGC GGCGTGCCTA ATACATGCAA 61 GTCGAGCGGA CAGATGGGAG CTTGCTCCCT GATGTTAGCG GCGGACGGGT GAGTAACACG 121 TGGGTAACCT GCCTGTAAGA CTGGGATAAC TCCGGGAAAC CGGGGCTAAT ACCGGATGCT 181 TGTTTGAACC GCATGGTTCA AACATAAAAG GTGGCTTCGG CTACCACTTA CAGATGGACC 241 CGCGGCGCAT TAGCTAGTTG GTGAGGTAAC GGCTCACCAA GGCAACGATG CGTAGCCGAC 301 CTGAGAGGGT GATCGGCCAC ACTGGGACTG AGACACGGCC CAGACTCCTA CGGGAGGCAG 361 CAGTAGGGAA TCTTCCGCAA TGGACGAAAG TCTGACGGAG CAACGCCGCG TGAGTGATGA 421 AGGTTTTCGG ATCGTAAAGC TCTGTTGTTA GGGAAGAACA AGTACCGTTC GAATAGGGCG 481 GTACCTTGAC GGTACCTAAC CAGAAAGCCA CGGCTAACTA CGTGCCAGCA GCCGCGGTAA 541 TACGTAGGTG GCAAGCGTTG TCCGGAATTA TTGGGCGTAA AGGGCTCGCA GGCGGTTCCT 601 TAAGTCTGAT GTGAAAGCCC CCGGCTCAAC CGGGGAGGGT CATTGGAAAC TGGGGAACTT 661 GAGTGCAGAA GAGGAGAGTG GAATTCCACG TGTAGCGGTG AAATGCGTAG AGATGTGGAG 721 GAACACCAGT GGCGAAGGCG ACTCTCTGGT CTGTAACTGA CGCTGAGGAG CGAAAGCGTG 781 GGGAGCGAAC AGGATTAGAT ACCCTGGTAG TCCACG Desired Expression Range : 1002.55 ~ 102672.55 Number of expression-level intermediates : 16 |