UTR Library Designer Error

Template 5'-UTR Sequence (25NT) : 0521 ( Length should be 25NT ) ,     Additional Constraints for designing 5'-UTR sequence (25NT) : REDUCED ( Length should be 25NT )

Protein Coding Sequence (at least N-term 35NT) : 213 DESIREE MANOR ( Length should be more than 35NT )

16s rRNA Sequence : FTP

Desired Expression Range : 52021.00 ~ 521.00 ( Desired max expression should be larger than that of min )

Number of expression-level intermediates : 64